Man page - cnvkit-reference(1)

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CNVKIT_REFERENCE

NAME
DESCRIPTION
positional arguments:
options:
To construct a generic, flat" copy number reference with neutral expectedcoverage:"
To disable specific automatic bias corrections:

NAME

cnvkit_reference - Compile a coverage reference from the given files (normal samples).

DESCRIPTION

usage: cnvkit.py reference [-h] [-f FASTA] [-o FILENAME] [-c]
[--min-cluster-size NUM]

[-x {m,y,male,Male,f,x,female,Female}] [-y] [-t TARGETS] [-a ANTITARGETS] [--no-gc] [--no-edge] [--no-rmask] [references ...]

positional arguments:

references

Normal-sample target or antitarget .cnn files, or the directory that contains them.

options:

-h , --help

show this help message and exit

-f FASTA, --fasta FASTA

Reference genome, FASTA format (e.g. UCSC hg19.fa)

-o FILENAME, --output FILENAME

Output file name.

-c , --cluster

Calculate and store summary stats for clustered subsets of the normal samples with similar coverage profiles.

--min-cluster-size NUM

Minimum cluster size to keep in reference profiles. [Default: 4]

-x {m,y,male,Male,f,x,female,Female}, --sample-sex
{m,y,male,Male,f,x,female,Female}, -g
{m,y,male,Male,f,x,female,Female}, --gender
{m,y,male,Male,f,x,female,Female}

Specify the chromosomal sex of all given samples as male or female. (Default: guess each sample from coverage of X and Y chromosomes).

-y , --male-reference , --haploid-x-reference

Create a male reference: shift female samples’ chrX log-coverage by -1 , so the reference chrX average is -1 . Otherwise, shift male samples’ chrX by +1, so the reference chrX average is 0.

To construct a generic, flat" copy number reference with neutral expectedcoverage:"

-t TARGETS, --targets TARGETS

Target intervals (.bed or .list)

-a ANTITARGETS, --antitargets ANTITARGETS

Antitarget intervals (.bed or .list)

To disable specific automatic bias corrections:

--no-gc

Skip GC correction.

--no-edge

Skip edge-effect correction.

--no-rmask

Skip RepeatMasker correction.