Man page - bp_unflatten_seq(1)

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BP_UNFLATTEN_SEQ

NAME
SYNOPSIS
DESCRIPTION
COMMAND LINE ARGUMENTS
TODO
FEEDBACK
Mailing Lists
Reporting Bugs
AUTHOR

NAME

bp_unflatten_seq - unflatten a genbank or genbank-style feature file into a nested SeqFeature hierarchy

SYNOPSIS

bp_unflatten_seq.PLS -e 3 -gff ˜/cvs/bioperl-live/t/data/AE003644_Adh-genomic.gb
bp_unflatten_seq.PLS --detail ˜/cvs/bioperl-live/t/data/AE003644_Adh-genomic.gb
bp_unflatten_seq.PLS -i foo.embl --from embl --to chadoxml -o out.chado.xml
bp_unflatten_seq.PLS --notypemap --detail --to asciitree -ethresh 2 AE003644_Adh-genomic.gb

DESCRIPTION

This script will unflatten a genbank or genbank-style file of SeqFeatures into a nested hierarchy.

See Bio::SeqFeature::Tools::Unflattener

In a GenBank/EMBL representation, features are ’flat’ - for example, there is no link between an mRNA and a CDS, other than implicit links (eg via tags or via splice site coordinates) which may be hard to code for.

This is most easily illustrated with the default output format, asciitree

An unflattened genbank feature set may look like this ( AB077698 )

Seq: AB077698
databank_entry 1..2701[+]
gene
mRNA
CDS hCHCR-G 80..1144[+]
exon 80..1144[+]
five_prime_UTR 1..79[+]
located_sequence_feature 137..196[+]
located_sequence_feature 239..292[+]
located_sequence_feature 617..676[+]
located_sequence_feature 725..778[+]
three_prime_UTR 1145..2659[+]
polyA_site 1606..1606[+]
polyA_site 2660..2660[+]

Or like this (portion of AE003734 )

gene
mRNA CG3320-RA
CDS CG3320-PA 53126..54971[-]
exon 52204..53323[-]
exon 53404..53631[-]
exon 53688..53735[-]
exon 53798..53918[-]
exon 54949..55287[-]
mRNA CG3320-RB
CDS CG3320-PB 53383..54971[-]
exon 52204..53631[-]
exon 53688..53735[-]
exon 53798..53918[-]
exon 54949..55287[-]

The unflattening will also ’normalize’ the containment hierarchy (in the sense of standardising it - e.g. making sure there is always a transcript record, even if genbank just specifies CDS and gene)

By default, the GenBank types will be mapped to SO types

See Bio::SeqFeature::Tools::TypeMapper

COMMAND LINE ARGUMENTS

-i|input FILE

input file (can also be specified as last argument)

-from FORMAT

input format (defaults to genbank)

probably doesn’t make so much sense to use this for non-flat formats; ie other than embl/genbank

-to FORMAT

output format (defaults to asciitree)

should really be a format that is nested SeqFeature aware; I think this is only asciitree, chadoxml and gff3

-gff

with export to GFF3 format (pre-3 GFFs make no sense with unflattened sequences, as they have no set way of representing feature graphs)

-o|output FILE

outfile defaults to STDOUT

-detail

show extra detail on features (asciitree mode only)

-e|ethresh INT

sets the error threshold on unflattening

by default this script will throw a wobbly if it encounters weird stuff in the genbank file - raise the error threshold to signal these to be ignored (and reported on STDERR )

-nomagic

suppress use_magic in unflattening (see Bio::SeqFeature::Tools::Unflattener

-notypemap

suppress type mapping (see Bio::SeqFeature::Tools::TypeMapper

TODO

Bio::SeqFeature::Tools::Unflattener allows fine-grained control over the unflattening process - need to add more options to allow this control at the command line

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web:

https://github.com/bioperl/bioperl-live/issues

AUTHOR

Chris Mungall E<lt>cjm-at-bioperl.orgE<gt>