Man page - seqan_tcoffee(1)

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Manual

SEQAN_TCOFFEE

NAME
SYNOPSIS
DESCRIPTION
OPTIONS
Main Options::
Segment Match Generation Options::
Scoring Options::
Guide Tree Options::
Alignment Evaluation Options::

NAME

seqan_tcoffee - Multiple sequence alignment

SYNOPSIS

seqan_tcoffee -s < FASTA FILE > [ OPTIONS ]

DESCRIPTION

SeqAn::T-Coffee is a multiple sequence alignment tool.

(c) Copyright 2009 by Tobias Rausch

OPTIONS

-h , --help

Display the help message.

--version

Display version information.

Main Options::

-s , --seq INPUT_FILE

Name of multi-fasta input file. Valid filetypes are: .frn , .fna , .ffn , .fasta , .faa , and .fa .

-a , --alphabet STRING

The used sequence alphabet. One of protein , dna , rna , and iupac . Default: protein .

-o , --outfile OUTPUT_FILE

Name of the output file. Valid filetypes are: .msf , .frn , .fna , .ffn , .fasta , .faa , and .fa . Default: out.fasta .

Segment Match Generation Options::

-m , --method List of STRING ’s

Defines the generation method for matches. To select multiple generation methods recall this option with different arguments. One of global , local , overlap , and lcs . Default: global and local .

-l , --libraries List of INPUT_FILE ’s

Name of match file. To select multiple files recall this option with different arguments. Valid filetypes are: .mums , .lib , .blast , and .aln .

-pa , --pairwise-alignment STRING

Pairwise alignment method. Default: unbanded for usual alignments (< 50 sequences), banded for deep alignments (>= 50 sequences) One of unbanded and banded .

-bw , --band-width INTEGER

Band width. This option automatically select banded pairwise alignment (see pa for details) In range [2..inf]. Default: 60 .

Scoring Options::

-g , --gop INTEGER

gap open penalty Default: -13 .

-e , --gex INTEGER

gap extension penalty Default: -1 .

-ma , --matrix STRING

score matrix Default: Blosum62 .

-ms , --msc INTEGER

match score Default: 5 .

-mm , --mmsc INTEGER

mismatch penalty Default: -4 .

Guide Tree Options::

-u , --usetree STRING

Name of the file containing the Newick Guide Tree .

-b , --build STRING

Method to build the tree. Following methods are provided: Neighbor-Joining ( nj ), UPGMA single linkage ( min ), UPGMA complete linkage ( max ), UPGMA average linkage ( avg ), UPGMA weighted average linkage ( wavg ). Neighbor-Joining creates an unrooted tree, which we root at the last joined pair. One of nj , min , max , avg , and wavg . Default: nj .

Alignment Evaluation Options::

-i , --infile INPUT_FILE

Name of the alignment file < FASTA FILE > Valid filetypes are: .frn , .fna , .ffn , .fasta , .faa , and .fa .