Man page - last-dotplot(1)
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Manual
LAST-DOTPLOT
NAMESYNOPSIS
DESCRIPTION
OPTIONS
NAME
last-dotplot - make a dotplot, a.k.a. Oxford Grid, of alignments in LAST tabular format
SYNOPSIS
last-dotplot
--help
last-dotplot
[
options
]
maf-or-psl-or-tab-alignments
last-dotplot
[
options
]
maf-or-psl-or-tab-alignments dotplot.png|gif|
...
DESCRIPTION
Draw a dotplot of pair-wise sequence alignments.
OPTIONS
-h , --help
show this help message and exit
-v , --verbose
show progress messages & data about the plot
-x INT, --width = INT
maximum width in pixels (default: 1000)
-y INT, --height = INT
maximum height in pixels (default: 1000)
-m M, --maxseqs = M
maximum number of horizontal or vertical sequences (default=100)
-1 PATTERN, --seq1 = PATTERN
which sequences to show from the 1st genome
-2 PATTERN, --seq2 = PATTERN
which sequences to show from the 2nd genome
--alignments = FILE
secondary alignments
--sort1 = N
genome1 sequence order: 0=input order, 1=name order, 2=length order, 3=alignment order (default=1)
--sort2 = N
genome2 sequence order: 0=input order, 1=name order, 2=length order, 3=alignment order (default=1)
--strands1 = N
genome1 sequence orientation: 0=forward orientation, 1=alignment orientation (default=0)
--strands2 = N
genome2 sequence orientation: 0=forward orientation, 1=alignment orientation (default=0)
--max-gap1 = FRAC
maximum unaligned (end,mid) gap in genome1: fraction of aligned length (default=1,4)
--max-gap2 = FRAC
maximum unaligned (end,mid) gap in genome2: fraction of aligned length (default=1,4)
--pad = FRAC
pad length when cutting unaligned gaps: fraction of aligned length (default=0.04)
-j N, --join = N
join: 0=nothing, 1=alignments adjacent in genome1, 2=alignments adjacent in genome2 (default=0)
--border-pixels = INT
number of pixels between sequences (default=1)
-a FILE, --bed1 = FILE , --rmsk1 = FILE , --genePred1 = FILE , --gap1 = FILE
read genome1 annotations
-b FILE, --bed2 = FILE , --rmsk2 = FILE , --genePred2 = FILE , --gap2 = FILE
read genome2 annotations
Text options:
-f FILE, --fontfile = FILE
TrueType or OpenType font file
-s SIZE, --fontsize = SIZE
TrueType or OpenType font size (default: 14)
--labels1 = N
genome1 labels: 0=name, 1=name:length, 2=name:start:length, 3=name:start-end (default=0)
--labels2 = N
genome2 labels: 0=name, 1=name:length, 2=name:start:length, 3=name:start-end (default=0)
--rot1 = ROT
text rotation for the 1st genome (default=h)
--rot2 = ROT
text rotation for the 2nd genome (default=v)
Color options:
-c COLOR, --forwardcolor = COLOR
color for forward alignments (default: red)
-r COLOR, --reversecolor = COLOR
color for reverse alignments (default: blue)
--border-color = COLOR
color for pixels between sequences (default=black)
--margin-color = COLOR
margin color
--exon-color = COLOR
color for exons (default=PaleGreen)
--cds-color = COLOR
color for protein-coding regions (default=LimeGreen)
--bridged-color = COLOR
color for bridged gaps (default: yellow)
--unbridged-color = COLOR
color for unbridged gaps (default: orange)