Man page - glam2(1)

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Manual

GLAM2

NAME
SYNOPSIS
DESCRIPTION
OPTIONS (DEFAULT SETTINGS)
SEE ALSO
REFERENCE
AUTHORS
COPYRIGHT

NAME

glam2 - Gapped Local Alignment of Motifs

SYNOPSIS

glam2 [ options ] alphabet my_seqs.fa

An alphabet other than p or n is interpreted as the name of an alphabet file.

DESCRIPTION

GLAM2 is a software package for finding motifs in sequences, typically amino-acid or nucleotide sequences. A motif is a re-occurring sequence pattern: typical examples are the TATA box and the CAAX prenylation motif. The main innovation of GLAM2 is that it allows insertions and deletions in motifs.

OPTIONS (DEFAULT SETTINGS)

-h

Show all options and their default settings.

-o

Output file ( stdout ).

-r

Number of alignment runs ( 10 ).

-n

End each run after this many iterations without improvement ( 10000 ).

-2

Examine both strands - forward and reverse complement.

-z

Minimum number of sequences in the alignment ( 2 ).

-a

Minimum number of aligned columns ( 2 ).

-b

Maximum number of aligned columns ( 50 ).

-w

Initial number of aligned columns ( 20 ).

-d

Dirichlet mixture file.

-D

Deletion pseudocount ( 0.1 ).

-E

No-deletion pseudocount ( 2.0 ).

-I

Insertion pseudocount ( 0.02 ).

-J

No-insertion pseudocount ( 1.0 ).

-q

Weight for generic versus sequence-set-specific residue abundances ( 1e+99 ).

-t

Initial temperature ( 1.2 ).

-c

Cooling factor per n iterations ( 1.44 ).

-u

Temperature lower bound ( 0.1 ).

-p

Print progress information at each iteration.

-m

Column-sampling moves per site-sampling move ( 1.0 ).

-x

Site sampling algorithm: 0 =FAST 1 =SLOW 2 =FFT ( 0 ).

-s

Seed for pseudo-random numbers ( 1 ).

SEE ALSO

glam2format (1), glam2mask (1), glam2-purge (1), glam2scan (1)

The full Hypertext documentation of GLAM2 is available online at http://bioinformatics.org.au/glam2/ or on this computer in /usr/share/doc/glam2/ .

REFERENCE

If you use GLAM2, please cite: MC Frith, NFW Saunders, B Kobe, TL Bailey (2008) Discovering sequence motifs with arbitrary insertions and deletions, PLoS Computational Biology (in press).

AUTHORS

Martin Frith

Author of GLAM2.

Timothy Bailey

Author of GLAM2.

Charles Plessy <plessy@debian.org>

Formatted this manpage in DocBook XML for the Debian distribution.

COPYRIGHT

The source code and the documentation of GLAM2 are released in the public domain.