--island-gap-size= <number> Alignment gaps above this size in nucleotides are considered to be islands [20]

--profile= <file> (Not yet implemented) Read an existing sequence alignment in XMFA format and align it to other sequences or alignments

--apply-backbone= <file> Read an existing sequence alignment in XMFA format and apply backbone statistics to it

--disable-backbone Disable backbone detection

--mums Find MUMs only, do not attempt to determine locally collinear blocks (LCBs)

--seed-weight= <number> Use the specified seed weight for calculating initial anchors

--backbone-output= <file> Backbone output file name (optional).

--match-input= <file> Use specified match file instead of searching for matches

--input-id-matrix= <file> An identity matrix describing similarity among all pairs of input sequences/alignments

--max-gapped-aligner-length= <number> Maximum number of base pairs to attempt aligning with the gapped aligner

--input-guide-tree= <file> A phylogenetic guide tree in NEWICK format that describes the order in which sequences will be aligned

--output-guide-tree= <file> Write out the guide tree used for alignment to a file

--version Display software version information

--debug Run in debug mode (perform internal consistency checks--very slow)

--collinear Assume that input sequences are collinear--they have no rearrangements

--no-weight-scaling Don’t scale LCB weights by conservation distance and breakpoint distance

--skip-refinement Do not perform iterative refinement

--skip-gapped-alignment Do not perform gapped alignment

--bp-dist-estimate-min-score= <number> Minimum LCB score for estimating pairwise breakpoint distance

--mem-clean Set this to true when debugging memory allocations

--gap-open= <number> Gap open penalty

--gap-extend= <number> Gap extend penalty

--substitution-matrix= <file> Nucleotide substitution matrix in NCBI format

--weight= <number> Minimum pairwise LCB score

--min-scaled-penalty= <number> Minimum breakpoint penalty after scaling the penalty by expected divergence

--hmm-p-go-homologous= <number> Probability of transitioning from the unrelated to the homologous state [0.00001]

--hmm-p-go-unrelated= <number> Probability of transitioning from the homologous to the unrelated state [0.000000001]

--hmm-identity= <number> Expected level of sequence identity among pairs of sequences, ranging between 0 and 1 [0.7]

--seed-family Use a family of spaced seeds to improve sensitivity

--solid-seeds Use solid seeds. Do not permit substitutions in anchor matches.

--coding-seeds Use coding pattern seeds. Useful to generate matches coding regions with 3rd codon position degeneracy.

--disable-cache Disable recursive anchor search cacheing to workaround a crash bug

--no-recursion Disable recursive anchor search