Man page - cufflinks(1)
Manual
CUFFLINKS
NAMESYNOPSIS
DESCRIPTION
General Options:
Advanced Abundance Estimation Options:
Advanced Assembly Options:
Advanced Reference Annotation Guided Assembly Options:
Advanced Program Behavior Options:
Supported library types:
Supported library normalization methods:
NAME
cufflinks - component of cufflinks suite
SYNOPSIS
cufflinks [ options ] <hits.sam>
DESCRIPTION
cufflinks v2.2.1 linked against Boost version 108300 -----------------------------
General Options:
-o /--output-dir
write all output files to this directory [ default: ./ ]
-p /--num-threads
number of threads used during analysis [ default: 1 ]
|
--seed |
value of random number generator seed [ default: 0 ] |
-G /--GTF
quantitate against reference transcript annotations
-g /--GTF-guide
use reference transcript annotation to guide assembly
-M /--mask-file
ignore all alignment within transcripts in this file
-b /--frag-bias-correct
use bias correction - reference fasta required [ default: NULL ]
-u /--multi-read-correct
use ’rescue method’ for multi-reads (more accurate) [ default: FALSE ]
--library-type
library prep used for input reads [ default: below ]
--library-norm-method
Method used to normalize library sizes [ default: below ]
Advanced Abundance Estimation Options:
-m /--frag-len-mean
average fragment length (unpaired reads only) [ default: 200 ]
-s /--frag-len-std-dev
fragment length std deviation (unpaired reads only) [ default: 80 ]
--max-mle-iterations
maximum iterations allowed for MLE calculation [ default: 5000 ]
--compatible-hits-norm
count hits compatible with reference RNAs only [ default: FALSE ]
--total-hits-norm
count all hits for normalization [ default: TRUE ]
--num-frag-count-draws
Number of fragment generation samples [ default: 100 ]
--num-frag-assign-draws
Number of fragment assignment samples per generation [ default: 50 ]
--max-frag-multihits
Maximum number of alignments allowed per fragment [ default: unlim ]
--no-effective-length-correction
No effective length correction [ default: FALSE ]
--no-length-correction
No length correction [ default: FALSE ]
-N /--upper-quartile-norm
Deprecated, use --library-norm-method [ DEPRECATED ]
--raw-mapped-norm
Deprecated, use --library-norm-method [ DEPRECATED ]
Advanced Assembly Options:
-L /--label
assembled transcripts have this ID prefix [ default: CUFF ]
-F /--min-isoform-fraction
suppress transcripts below this abundance level [ default: 0.10 ]
-j /--pre-mrna-fraction
suppress intra-intronic transcripts below this level [ default: 0.15 ]
-I /--max-intron-length
ignore alignments with gaps longer than this [ default: 300000 ]
-a /--junc-alpha
alpha for junction binomial test filter [ default: 0.001 ]
-A /--small-anchor-fraction
percent read overhang taken as ’suspiciously small’ [ default: 0.09 ]
--min-frags-per-transfrag
minimum number of fragments needed for new transfrags [ default: 10 ]
--overhang-tolerance
number of terminal exon bp to tolerate in introns [ default: 8 ]
--max-bundle-length
maximum genomic length allowed for a given bundle [ default:3500000 ]
--max-bundle-frags
maximum fragments allowed in a bundle before skipping [ default: 500000 ]
--min-intron-length
minimum intron size allowed in genome [ default: 50 ]
--trim-3-avgcov-thresh
minimum avg coverage required to attempt 3’ trimming [ default: 10 ]
--trim-3-dropoff-frac
fraction of avg coverage below which to trim 3’ end [ default: 0.1 ]
--max-multiread-fraction
maximum fraction of allowed multireads per transcript [ default: 0.75 ]
--overlap-radius
maximum gap size to fill between transfrags (in bp) [ default: 50 ]
Advanced Reference Annotation Guided Assembly Options:
--no-faux-reads
disable tiling by faux reads [ default: FALSE ]
--3-overhang-tolerance
overhang allowed on 3’ end when merging with reference[ default: 600 ]
--intron-overhang-tolerance
overhang allowed inside reference intron when merging [ default: 30 ]
Advanced Program Behavior Options:
-v /--verbose
log-friendly verbose processing (no progress bar) [ default: FALSE ]
-q /--quiet
log-friendly quiet processing (no progress bar) [ default: FALSE ]
--no-update-check
do not contact server to check for update availability[ default: FALSE ]
Supported library types:
ff-firststrand ff-secondstrand ff-unstranded fr-firststrand fr-secondstrand fr-unstranded (default) transfrags
Supported library normalization methods:
classic-fpkm